ICC/IF image of ab85195 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85195 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab85195, at 1/100 dilution, staining Apolipoprotein L 1 in formalin-fixed, paraffin-embedded human fetal liver by Immunohistochemistry.
All lanes : Anti-Apolipoprotein L 1 antibody (ab85195) at 1/500 dilutionLane 1 : Jurkat lysateLane 2 : HepG2 lysateLane 3 : Human fetal liver lysate