All lanes : Anti-Apoptosis repressor with CARD antibody (ab62149) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : KB whole cell lysateLane 3 : A549 whole cell lysateObserved band size : 25 kDa (why is the actual band size different from the predicted?)Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
ab62149 at 181 ug/ml, staining Human Apoptosis repressor with CARD in heart tissue by IHC-P.
ab62149 at 5µg/ml, staining Human Apoptosis repressor with CARD in skeletal muscle by IHC-P.