Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific)

• Supplier: Cell Signaling Technology
• Catalog: 9018
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7F10
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt1 protein.
• Description: Rabbit Monoclonal
• Gene: AKT1
• Conjugate: Akt1 Specific

Product images

Western blot analysis of extracts from LNCaP cells, transfected with a construct expressing Akt1 shRNA or Akt2 shRNA, uninduced (-) or doxycycline-induced for the indicated times, using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (top), Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific) #8599 (2nd from top), Akt1 (C73H10) Rabbit mAb #2938 (middle), Akt2 (D6G4) Rabbit mAb #3063 (2nd from bottom), or PI3 Kinase p85 (19H8) Rabbit mAb #4257 (bottom). (Data kindly provided by Drs. Rebecca Chin and Alex Toker, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA).

Western blot analysis of purified recombinant phospho-Akt1, phospho-Akt2 and phospho-Akt3 proteins using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (upper), Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (middle) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western blot analysis of extracts from Akt1 (-/-) mouse embryonic fibroblast (MEF) or Akt2 (-/-) MEF, untreated or stimulated with hPDGF #8913 (100 ng/ml, 15 min), using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (upper), Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (middle), or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Confocal immunofluorescent analysis of Akt1 (-/-) mouse embryonic fibroblast (MEF) (left column), or Akt2 (-/-) MEF (right column) cells, stimulated with hPDGF #8913 (100 ng/ml, 15 min) (top row) or inhibited with LY294002 #9901 (bottom row), using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Akt1 (-/-) mouse embryonic fibroblast (MEF) cells (blue) or Akt2 (-/-) MEF cells (green), PDGF-treated (100 ng/mL, 15 min), using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary Ab.