All lanes : Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : 293 cell lysateLane 4 : Hman cerebellum lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded, Human cervix carcinoma tissue labeling Aprataxin with ab192598 at a 1/1000 dilution. Counter stained with hematoxylin. Negative control also shown.
Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling Aprataxin with ab192598 at a 1/200 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.