Anti-Aquaporin 2 antibody - C-terminal
| Name | Anti-Aquaporin 2 antibody - C-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab85876 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide designed within the C-terminal region of Human Aquaporin 2, conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | AQP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab85876 staining Aquaporin 2 - C-terminal in Rat kidney tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 25% serum free protein block for 5 minutes at 25°C; antigen retrieval was by heat mediation with a Tris/EDTA (pH 9.0) buffer. Samples were incubated with primary antibody (1/5000 in serum free antibody diluent) for 30 minutes. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ICC/IF image of ab85876 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85876, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Origin of regenerating tubular cells after acute kidney injury. - Origin of regenerating tubular cells after acute kidney injury.
Berger K, Bangen JM, Hammerich L, Liedtke C, Floege J, Smeets B, Moeller MJ. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1533-8. doi:
In vivo and in vitro analysis of age-associated changes and somatic cellular - In vivo and in vitro analysis of age-associated changes and somatic cellular
Berkenkamp B, Susnik N, Baisantry A, Kuznetsova I, Jacobi C, Sorensen-Zender I, Broecker V, Haller H, Melk A, Schmitt R. PLoS One. 2014 Feb 4;9(2):e88071.