Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 4060
• Prices: $119.00, $318.00, $750.00
• Sizes: 20 µl (4 western blots), 100 µl (20 western blots), 300 µl (60 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D9E
• Applications: WB IP IHC-P IHC-F ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Hamster, Monkey, Drosophila, Zebrafish, Bovine, Chicken, Xenopus, Dog, Pig
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt.
• Description: Rabbit Monoclonal
• Gene: AKT1

Product images

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain ® Antibody Diluent #8112 (left) to TBST/5% normal goat serum (right) using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb.

Immunohistochemical analysis using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded PTEN heterozygous mutant mouse endometrium using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb. (Tissue section courtesy of Dr. Sabina Signoretti, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.)

Immunohistochemical analysis of paraffin-embedded U-87MG xenograft, untreated (left) or lambda phosphatase-treated (right), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb.

Immunohistochemical analysis of frozen SKOV3 xenograft using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb compared to a nonspecific negative control antibody (red).