All lanes : Anti-Archaemetzincin 2 antibody (ab59926) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (1:4) (serum free) Lane 2 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (1:4) (treated with TPA) Lane 3 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (1:4) (treated with IL1-beta) Lane 4 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (1:4) (treated with TNF-alpha) Lysates/proteins at 20 µl per lane.