All lanes : Anti-ARD1A antibody (ab155687) at 1/5000 dilutionLane 1 : H1299 cell lysate, transfected with scramble siRNA Lane 2 : H1299 cell lysate, transfected with ARDIA siRNA Lysates/proteins at 30 µg per lane.
All lanes : Anti-ARD1A antibody (ab155687) at 1/1000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL1 whole cell lysateLane 4 : C2C12 whole cell lysateLane 5 : RAW264.7 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of methanol-fixed HeLa cells (100% for 5 mins) labeling ARD1A with ab155687 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue labeling ARD1A with ab155687 at 1/500 dilution.