All lanes : Anti-ARF1 antibody (ab183576) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U2 OS cell lysateLane 3 : 3T3 cell lysateLane 4 : NRK cell lysateLane 5 : MDCK cell lysateLysates/proteins at 25 µg per lane.Secondarygoat anti-rabbit IgG-HRP at 1/1500 dilutiondeveloped using the ECL technique
Detection of ARF1 in Immunoprecipitates of HeLa cell lysate. Antigen-antibody complexes were formed by incubating 500μg of HeLa whole cell lysate with 2μg ab183576 (lane 2) compared with HeLa cell lysate as a positive control (lane 1). For detection, ab183576 was used at 1/1000 dilution.
Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling ARF1 with ab183576 at 1/100 dilution followed with DyLight 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.