Anti-ARF1 antibody [ARFS 3F1]
| Name | Anti-ARF1 antibody [ARFS 3F1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11038 |
| Prices | $398.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | ARFS 3F1 |
| Applications | IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: SNQLRNQ , corresponding to C terminal amino acids 174-180 of Human ARF1 |
| Description | Mouse Monoclonal |
| Gene | ARF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Anti-mouse HRP (ab6728) (1/1000) was used as the secondary antibody. The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight)See Abreview
IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab11038 staining ARF1 in the Panc-1 cell line from Human pancreatic carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formalddehyde and blocked with 1% Donkey serum in PBST for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% Donkey serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Product References
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Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of - Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of
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Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate - Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate
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Fbx8 makes Arf6 refractory to function via ubiquitination. - Fbx8 makes Arf6 refractory to function via ubiquitination.
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