Anti-ARF1 antibody [EP442Y]
| Name | Anti-ARF1 antibody [EP442Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32524 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP442Y |
| Applications | WB IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in human ARF1 |
| Description | Rabbit Monoclonal |
| Gene | ARF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-ARF1 antibody [EP442Y] (ab32524) at 1/2000 dilution + 293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8960_ab32524wb.gif)
Anti-ARF1 antibody [EP442Y] (ab32524) at 1/2000 dilution + 293 cell lysate
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution.
Overlay histogram showing HeLa cells stained with ab32524 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32524, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Skeletal muscle perilipin 3 and coatomer proteins are increased following - Skeletal muscle perilipin 3 and coatomer proteins are increased following
Covington JD, Galgani JE, Moro C, LaGrange JM, Zhang Z, Rustan AC, Ravussin E, Bajpeyi S. PLoS One. 2014 Mar 14;9(3):e91675.
CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and - CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and
Schultz ML, Tecedor L, Stein CS, Stamnes MA, Davidson BL. PLoS One. 2014 May 2;9(5):e96647.
AMF-26, a novel inhibitor of the Golgi system, targeting ADP-ribosylation factor - AMF-26, a novel inhibitor of the Golgi system, targeting ADP-ribosylation factor
Ohashi Y, Iijima H, Yamaotsu N, Yamazaki K, Sato S, Okamura M, Sugimoto K, Dan S, Hirono S, Yamori T. J Biol Chem. 2012 Feb 3;287(6):3885-97.
Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds - Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds
Arita M, Kojima H, Nagano T, Okabe T, Wakita T, Shimizu H. J Virol. 2011 Mar;85(5):2364-72.
Small G proteins in peroxisome biogenesis: the potential involvement of - Small G proteins in peroxisome biogenesis: the potential involvement of
Anthonio EA, Brees C, Baumgart-Vogt E, Hongu T, Huybrechts SJ, Van Dijck P, Mannaerts GP, Kanaho Y, Van Veldhoven PP, Fransen M. BMC Cell Biol. 2009 Aug 17;10:58.
p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of - p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of
Luo W, Wang Y, Reiser G. J Biol Chem. 2007 Oct 12;282(41):30246-55. Epub 2007 Aug 10.