Anti-ARF1 antibody [EPR443]

Name	Anti-ARF1 antibody [EPR443]
Supplier	Abcam
Catalog	ab108347
Prices	$387.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR443
Applications	WB IHC-P ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide corresponding to residues in Human ARF1
Description	Rabbit Monoclonal
Gene	ARF1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-ARF1 antibody [EPR443] (ab108347) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal brain lysateLane 3 : MCF7 lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.

Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.

Product References

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and - CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and

Schultz ML, Tecedor L, Stein CS, Stamnes MA, Davidson BL. PLoS One. 2014 May 2;9(5):e96647.

Secretion of soluble vascular endothelial growth factor receptor 1 - Secretion of soluble vascular endothelial growth factor receptor 1

Jung JJ, Tiwari A, Inamdar SM, Thomas CP, Goel A, Choudhury A. PLoS One. 2012;7(9):e44572.