All lanes : Anti-ARF4 antibody [EPR12133(B)] (ab171746) at 1/10000 dilutionLane 1 : HT-29 cell lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1X PBS (negative control) using ab171746 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry of paraffin-embedded Human testis tissue labeling ARF4 using ab171746 at 1/100 dilution.
Immunohistochemistry of paraffin-embedded Human kidney tissue labeling ARF4 using ab171746 at 1/100 dilution.
Immunofluorescence analysis of HepG2 cells labeling ARF4 using ab171746 at 1/100 dilution (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized K562 cells labeling ARF4 using ab171746 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).