Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot®:Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling ARFIP2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling ARFIP2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling ARFIP2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-ARFIP2 antibody [EPR11943] (ab180503) at 1/5000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : MCF-7 cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution