Anti-Arg 3.1 antibody
| Name | Anti-Arg 3.1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85656 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Mouse, Rat, Goat, Chicken, Guinea Pig, Bovine |
| Antigen | Synthetic peptide: LDLPQKQGEP LDQFLWRKRD LYQTLYVDAD EEEIIQYVVG TLQPKLKRFL , corresponding to internal sequence amino acids 288-337 of Human Arg 3 |
| Blocking Peptide | Arg 3.1 peptide |
| Description | Rabbit Polyclonal |
| Gene | ARC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Arg 3.1 antibody (ab85656) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
IHC image of ab85656 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85656, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adrenal tissue labelling Arg 3.1 with ab85656 at 5µg/ml.