Anti-ARHGAP18 antibody (ab175970) at 1/1000 dilution + MDA-MB231 cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling ARHGAP18 using ab175970 at a 1/50 dilution, followed by peroxidase-conjugation to the secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB231 cells labeling ARHGAP18 using ab175970 at a 1/10 dilution (bottom histogram), compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.