All lanes : Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilutionLane 1 : Daudi cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution + Ramos cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.