Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab122345 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122345, at a 1/150 dilution, staining ARHGEF38 in paraffin-embedded Human skin tissue by immunohistochemistry.
All lanes : Anti-ARHGEF38 antibody (ab122345) at 1/250 dilutionLane 1 : Vector only transfected HEK293T lysateLane 2 : ARHGEF38-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))developed using the ECL technique