All lanes : Anti-ARL15 antibody [EPR12591] (ab178425) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : K562 lysateLane 3 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of K562 cells labeling ARL15 with ab178425 at 1/50 dilution (red). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178425 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.