Anti-Aromatase antibody
| Name | Anti-Aromatase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18995 |
| Prices | $391.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine, Pig |
| Antigen | Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924 |
| Blocking Peptide | Aromatase peptide |
| Description | Rabbit Polyclonal |
| Gene | CYP19A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab18995 staining Human normal placenta. Staining is localised to cell membrane.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-Aromatase antibody (ab18995) at 1 µg/ml + Brain (Human) Membrane Lysate - adult normal tissue (ab29456) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Occult microscopic endometriosis: undetectable by laparoscopy in normal - Occult microscopic endometriosis: undetectable by laparoscopy in normal
Khan KN, Fujishita A, Kitajima M, Hiraki K, Nakashima M, Masuzaki H. Hum Reprod. 2014 Mar;29(3):462-72.
The steroid hormone environment during primordial follicle formation in perinatal - The steroid hormone environment during primordial follicle formation in perinatal
Dutta S, Mark-Kappeler CJ, Hoyer PB, Pepling ME. Biol Reprod. 2014 Sep;91(3):68.
Genetic factors modulate the impact of pubertal androgen excess on insulin - Genetic factors modulate the impact of pubertal androgen excess on insulin
Dowling AR, Nedorezov LB, Qiu X, Marino JS, Hill JW. PLoS One. 2013 Nov 20;8(11):e79849.
AIB1:ERalpha transcriptional activity is selectively enhanced in aromatase - AIB1:ERalpha transcriptional activity is selectively enhanced in aromatase
O'Hara J, Vareslija D, McBryan J, Bane F, Tibbitts P, Byrne C, Conroy RM, Hao Y, Gaora PO, Hill AD, McIlroy M, Young LS. Clin Cancer Res. 2012 Jun 15;18(12):3305-15.
Inhibition of estradiol synthesis attenuates renal injury in male - Inhibition of estradiol synthesis attenuates renal injury in male
Manigrasso MB, Sawyer RT, Marbury DC, Flynn ER, Maric C. Am J Physiol Renal Physiol. 2011 Sep;301(3):F634-40. doi:
Androgen-induced progression of arterial calcification in apolipoprotein E-null - Androgen-induced progression of arterial calcification in apolipoprotein E-null
McRobb L, Handelsman DJ, Heather AK. Endocrinology. 2009 Feb;150(2):841-8.
De novo synthesis of estrogen in pregnant uterus is critical for stromal - De novo synthesis of estrogen in pregnant uterus is critical for stromal
Das A, Mantena SR, Kannan A, Evans DB, Bagchi MK, Bagchi IC. Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12542-7. doi:
The androgen metabolite 5alpha-androstane-3beta,17beta-diol (3betaAdiol) induces - The androgen metabolite 5alpha-androstane-3beta,17beta-diol (3betaAdiol) induces
Sikora MJ, Cordero KE, Larios JM, Johnson MD, Lippman ME, Rae JM. Breast Cancer Res Treat. 2009 May;115(2):289-96.
Enhanced expression of aromatase in p53-inactivated mammary epithelial cells. - Enhanced expression of aromatase in p53-inactivated mammary epithelial cells.
Choi HK, Roh SH, Kim HG, Han EH, Jeong HG, Kang KW. Endocr Relat Cancer. 2008 Mar;15(1):139-47.
Liver receptor homolog 1 is essential for ovulation. - Liver receptor homolog 1 is essential for ovulation.
Duggavathi R, Volle DH, Mataki C, Antal MC, Messaddeq N, Auwerx J, Murphy BD, Schoonjans K. Genes Dev. 2008 Jul 15;22(14):1871-6.