Anti-Aromatase antibody
| Name | Anti-Aromatase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab35604 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-F IHC-P |
| Species Reactivities | Dog, Human, Rabbit |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase |
| Description | Rabbit Polyclonal |
| Gene | CYP19A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Aromatase antibody (ab35604) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.See Abreview
ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.
Product References
Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian - Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian
Laws MJ, Kannan A, Pawar S, Haschek WM, Bagchi MK, Bagchi IC. PLoS Genet. 2014 Mar 6;10(3):e1004230.
The steroid hormone environment during primordial follicle formation in perinatal - The steroid hormone environment during primordial follicle formation in perinatal
Dutta S, Mark-Kappeler CJ, Hoyer PB, Pepling ME. Biol Reprod. 2014 Sep;91(3):68.
Aromatase is increased in astrocytes in the presence of elevated pressure. - Aromatase is increased in astrocytes in the presence of elevated pressure.
Gatson JW, Simpkins JW, Yi KD, Idris AH, Minei JP, Wigginton JG. Endocrinology. 2011 Jan;152(1):207-13.
Estrogen receptors in the central auditory system of male and female mice. - Estrogen receptors in the central auditory system of male and female mice.
Charitidi K, Canlon B. Neuroscience. 2010 Feb 3;165(3):923-33.
Concomitant mutations in the P450 oxidoreductase and androgen receptor genes - Concomitant mutations in the P450 oxidoreductase and androgen receptor genes
Idkowiak J, Malunowicz EM, Dhir V, Reisch N, Szarras-Czapnik M, Holmes DM, Shackleton CH, Davies JD, Hughes IA, Krone N, Arlt W. J Clin Endocrinol Metab. 2010 Jul;95(7):3418-27.
Chlamydia trachomatis infection of human trophoblast alters estrogen and - Chlamydia trachomatis infection of human trophoblast alters estrogen and
Azenabor AA, Kennedy P, Balistreri S. Int J Med Sci. 2007 Sep 6;4(4):223-31.