Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling Arp3 using ab181164 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunofluorescence analysis of -20°C Acetone fixed HeLa cells labeling Arp3 with ab181164 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Arp3 with ab181164 at a 1/250 dilution. Prediluted HRP Rabbit IgG secondary was used. Counterstained with Hematoxylin.
All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLane 3 : A549 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution