Anti-Artemis antibody
| Name | Anti-Artemis antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab35649 |
| Prices | $359.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human Artemis |
| Description | Rabbit Polyclonal |
| Gene | DCLRE1C |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Artemis antibody (ab35649) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab35649 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35649, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA - RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA
Ho SR, Mahanic CS, Lee YJ, Lin WC. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2646-55. doi:
Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon - Endonuclease G initiates DNA rearrangements at the MLL breakpoint cluster upon
Gole B, Baumann C, Mian E, Ireno CI, Wiesmuller L. Oncogene. 2014 Aug 18.
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
Sousa MM, Zub KA, Aas PA, Hanssen-Bauer A, Demirovic A, Sarno A, Tian E, Liabakk NB, Slupphaug G. PLoS One. 2013;8(2):e55493.