Anti-Asialoglycoprotein Receptor 2 antibody [EPR16974]

• Supplier: Abcam
• Catalog: ab197032
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16974
• Applications: WB IHC-P IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Asialoglycoprotein Receptor 2 aa 1-100
• Description: Rabbit Monoclonal
• Gene: ASGR2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Asialoglycoprotein Receptor 2 antibody [EPR16974] (ab197032) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysateLane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 5 : HeLa (Human epithetial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-Asialoglycoprotein Receptor 2 antibody [EPR16974] (ab197032) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Asialoglycoprotein Receptor 2 with ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cell membrane staining on Human liver tissue is observed; Uniprot suggests Cell membrane as subcellular location. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue (negative control tissue) using  ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Asialoglycoprotein Receptor 2 was immunoprecipitated from Human fetal liver with ab197032 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197032 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human fetal liver. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab197032 in Human fetal liver.Blocking and dilution buffer and concentration: 5% NFDM/TBST.