Anti-Asialoglycoprotein Receptor 2 antibody [EPR16975]

• Supplier: Abcam
• Catalog: ab200196
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16975
• Applications: IP IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Asialoglycoprotein Receptor 2 aa 200-300
• Description: Rabbit Monoclonal
• Gene: ASGR2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Asialoglycoprotein Receptor 2 antibody [EPR16975] (ab200196) at 1/5000 dilutionLane 1 : Human fetal liver lysateLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Asialoglycoprotein Receptor 2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Cytoplasm and cell membrane staining on Human liver tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Asialoglycoprotein Receptor 2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.No staining on Human skeletal muscle tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Asialoglycoprotein Receptor 2 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab200196 at 1/30 dilution.Western blot was performed from the immunoprecipitate using ab200196 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal liver whole cell lysate 10 µg (Input).Lane 2: ab200196 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200196 in Human fetal liver whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.