Western blot analysis of extracts from LNCaP cells, untreated, serum-starved, or Torin-treated as indicated, using Phospho-Akt (Thr450) (D5G4) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Western blot analysis of extracts from various cells lines using Phospho-Akt (Thr450) (D5G4) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Immunoprecipitation of Phopho-Akt (Thr450) from MEF cell extracts, using Normal Rabbit IgG #2729 (lane 2) or Phospho-Akt (Thr450) (D5G4) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Akt (Thr450) (D5G4) Rabbit mAb.