Anti-ATE1 antibody [EPR13667(2)] - N-terminal

• Supplier: Abcam
• Catalog: ab199423
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13667(2)
• Applications: IHC-P WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATE1 aa 1-100
• Description: Rabbit Monoclonal
• Gene: ATE1
• Conjugate: Unconjugated

Product images

All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution + Human fetal liver tissue lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Mouse spleen tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysateLane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 4 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysateLane 5 : Mouse kidney tissue lysateLane 6 : Rat kidney tissue lysateLane 7 : Rat spleen tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Rat kidney tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATE1 with ab199423 at 1/220 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.