Phospho-Akt (Ser473) (193H12) Rabbit mAb
| Name | Phospho-Akt (Ser473) (193H12) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 4058 |
| Prices | $109.00, $307.00, $719.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 193H12 |
| Applications | WB IP ICC/IF FC |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt. |
| Description | Rabbit Monoclonal |
| Gene | AKT1 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from untreated or PDGF-treated NIH/3T3 cells, pretreated with wortmannin #9951 and/or rapamycin #9904 as indicated, using Phospho-Akt (Ser473) (193H12) Rabbit mAb (upper) or Akt Antibody #9272 (lower).
Confocal immunofluorescent analysis of C2C12 cells, U0126/LY294002/Rapamycin-treated (left) or insulin-treated (right) using Phospho-Akt (Ser473) (193H12) Rabbit mAb (green). Actin filaments were labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 #9901 and Wortmannin #9951 treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb compared to a nonspecific negative control antibody (red).