Anti-ATF3 antibody [44C3a]

• Supplier: Abcam
• Catalog: ab58668
• Prices: $390.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 44C3a
• Applications: WB DB ICC/IF ICC/IF IHC-F IHC-P
• Species Reactivities: Rat, Human
• Antigen: Recombinant fragment of Human ATF3
• Description: Mouse Monoclonal
• Gene: ATF3
• Conjugate: Unconjugated

Product images

Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein

ab58668 staining ATF3 in A549 cells treated with ionomycin Ca2+ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca2+ salt, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca2+ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Product References

Satellite glial cells surrounding primary afferent neurons are activated and - Satellite glial cells surrounding primary afferent neurons are activated and

Nascimento DS, Castro-Lopes JM, Moreira Neto FL. PLoS One. 2014 Sep 23;9(9):e108152.

Differential effects of hypoxic stress in alveolar epithelial cells and - Differential effects of hypoxic stress in alveolar epithelial cells and

Signorelli S, Jennings P, Leonard MO, Pfaller W. Cell Physiol Biochem. 2010;25(1):135-44.

Inter-laboratory comparison of human renal proximal tubule (HK-2) transcriptome - Inter-laboratory comparison of human renal proximal tubule (HK-2) transcriptome

Jennings P, Aydin S, Bennett J, McBride R, Weiland C, Tuite N, Gruber LN, Perco P, Gaora PO, Ellinger-Ziegelbauer H, Ahr HJ, Kooten CV, Daha MR, Prieto P, Ryan MP, Pfaller W, McMorrow T. Toxicol In Vitro. 2009 Apr;23(3):486-99.