Anti-ATG2B antibody
| Name | Anti-ATG2B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab116215 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 117-146 of Human ATG2B (NP_060506 |
| Description | Rabbit Polyclonal |
| Gene | ATG2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-ATG2B antibody (ab116215) at 1/100 dilution + CEM cell line lysate at 35 µg
ab116215 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116215 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.