Anti-ATL3 antibody

• Supplier: Abcam
• Catalog: ab104262
• Prices: $370.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Hamster, Human
• Antigen: Synthetic peptide corresponding to a region within internal sequence amino acids 230-260 of Human ATL3, conjugated to KLH
• Description: Rabbit Polyclonal
• Gene: ATL3
• Conjugate: Unconjugated

Product images

ICC/IF image of ab104262 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104262 at 0.2µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-ATL3 antibody (ab104262) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

Anti-ATL3 antibody (ab104262) at 1/100 dilution + CHO cell lysate at 35 µg

Product References

Definitive evidence using enucleated cytoplasts for a nongenomic basis for the - Definitive evidence using enucleated cytoplasts for a nongenomic basis for the

Lee JE, Yang YM, Yuan H, Sehgal PB. Am J Physiol Cell Physiol. 2013 Feb 15;304(4):C312-23. doi:

Nongenomic STAT5-dependent effects on Golgi apparatus and endoplasmic reticulum - Nongenomic STAT5-dependent effects on Golgi apparatus and endoplasmic reticulum

Lee JE, Yang YM, Liang FX, Gough DJ, Levy DE, Sehgal PB. Am J Physiol Cell Physiol. 2012 Mar 1;302(5):C804-20. doi: