Anti-ATP citrate lyase antibody [EP704Y]
| Name | Anti-ATP citrate lyase antibody [EP704Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40793 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP704Y |
| Applications | ICC/IF ICC/IF IHC-P WB FC IP |
| Species Reactivities | Mouse, Rat, Human, Marmoset |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ATP citrate lyase aa 1050-1150 (C terminal) |
| Description | Rabbit Monoclonal |
| Gene | ACLY |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution + Hela cell lysate.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10932_ab40793wb.jpg)
Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution + Hela cell lysate.
![AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Rabbit monoclonal [Y308] to AMPK gamma 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 122kDa: ATP citrate lyase; non specific - 60kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10933_ATP-citrate-lyase-Primary-antibodies-ab40793-3.jpg)
AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Rabbit monoclonal [Y308] to AMPK gamma 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 122kDa: ATP citrate lyase; non specific - 60kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab40793 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40793, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLa cells stained with ab40793 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40793, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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