Anti-ATP5L2 + ATP5L [EPR15636] antibody

• Supplier: Abcam
• Catalog: ab191417
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15636
• Applications: ICC/IF ICC/IF IP WB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment within Human ATP5L2 + ATP5L aa 1 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: ATP5L
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).Negative controls: anti-ATP5L2 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. 

All lanes : Anti-ATP5L2 + ATP5L [EPR15636] antibody (ab191417) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Human fetal liver tissue lysateLane 3 : Human pancreas tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19.4 μg/ml)  followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.Inset: Negative control: using PBS instead of primary antibody.

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab191417 at 1/100 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.