Anti-ATP5O antibody [4C11C10D12]

Name	Anti-ATP5O antibody [4C11C10D12]
Supplier	Abcam
Catalog	ab110276
Prices	$391.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	4C11C10D12
Applications	IHC-F ICC/IF ICC/IF WB FC IHC-P
Species Reactivities	Bovine, Human, Monkey
Antigen	Recombinant mature Human ATP5O
Description	Mouse Monoclonal
Gene	ATP5O
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mlLane 1 : Human heart mitochondria at 5 µgLane 2 : Bovine heart mitochondria at 1 µg

Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mlLane 1 : HDFn (human) cell lysates at 20 µgLane 2 : COS7 (monkey) cell lysates at 20 µgLane 3 : H4IIE (rat) cell lysates at 20 µgLane 4 : MEF (mouse) cell lysates at 20 µgLane 5 : bovine heart mitochondria lysates at 5 µg

Product References

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