All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Y79 cell lysateLane 3 : 293T cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution.
Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution
Flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green).
Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution.
Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.