Anti-Axl antibody
| Name | Anti-Axl antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37861 |
| Prices | $391.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P FC IHC-F WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to Human Axl aa 21-37 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | AXL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Axl antibody (ab37861) + SKBR3 cell lysateSecondaryHRP-conjugated anti-rabbit IgGdeveloped using the ECL techniqueObserved band size : 97 kDa (why is the actual band size different from the predicted?)Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer (BC) tissue labelling Axl with ab37861. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.
ab37861 staining Axl in human breast tumor by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween20 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with the primary antibody (in PBS + 10% serum) for 12 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated goat anti-rabbit IgG monoclonal was used as the secondary antibody.See Abreview
ICC/IF image of ab37861 stained SW480 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37861 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Flow cytometry analysis of WiDr cells labelling Axl (green) with ab37861 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Product References
Expression of growth arrest-specific protein 6 and Axl molecules in the left - Expression of growth arrest-specific protein 6 and Axl molecules in the left
Lee CH, Shieh YS, Tsai CS, Hung YJ, Tsai YT, Lin CY. J Clin Pathol. 2014 Jun;67(6):506-11.
High glucose induces human endothelial dysfunction through an Axl-dependent - High glucose induces human endothelial dysfunction through an Axl-dependent
Lee CH, Shieh YS, Hsiao FC, Kuo FC, Lin CY, Hsieh CH, Hung YJ. Cardiovasc Diabetol. 2014 Feb 26;13:53.
Plasma concentrations predict aortic expression of growth-arrest-specific protein - Plasma concentrations predict aortic expression of growth-arrest-specific protein
Lee CH, Shieh YS, Tsai CS, Hung YJ, Tsai YT, Lin CY. PLoS One. 2013 Nov 13;8(11):e79452.