All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression)Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression)Lane 3 : Whole cell lysate of non-infected MCF7Lysates/proteins at 10 µg per lane.SecondaryAn HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
