ab89224 used in Flow Cytometry.Cells were trypsinized for 1 minute at room temperature and wahed once in cold PBS. They were stained with ab89224 diluted 1/200 in 5% BSA/PBS for 45 minutes on ice, washed and incbuated with ab98794 at a 1/200 dilution. Cells were filtered and read in a FACScan using the FL1 sensor.
ab89224 staining Axl in african green monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde and then blocked using 5% serum for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 546 (red) used at a 1/400 dilution. DAPI was used to counterstain nuclei.