Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade
| Name | Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab76009 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR2204Y |
| Applications | ChIP ICC/IF ICC/IF FC WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A phospho specific peptide corresponding to residues surrounding threonine 487 of human B MyB |
| Description | Rabbit Monoclonal |
| Gene | MYBL2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009) at 1/1000 dilutionLane 1 : Raji cell lysate, untreatedLane 2 : Raji cell lysate treated with TPA + IGFLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/11853_ab76009-1.jpg)
All lanes : Anti-B MyB (phospho T487) antibody [EPR2204Y] - ChIP Grade (ab76009) at 1/1000 dilutionLane 1 : Raji cell lysate, untreatedLane 2 : Raji cell lysate treated with TPA + IGFLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution
ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.
ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.
Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent - B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent
Werwein E, Dzuganova M, Usadel C, Klempnauer KH. Cell Death Dis. 2013 Feb 28;4:e511.
The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene - The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene
Sadasivam S, Duan S, DeCaprio JA. Genes Dev. 2012 Mar 1;26(5):474-89.