Flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling BAAT1 with ab181868 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-BAAT1 antibody [EPR13753-43] - C-terminal (ab181868) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181868 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.