Western blot analysis of extracts from CHO cells, transfected with non-targeted (-) or SignalSilence ® Akt siRNA I (+) siRNA, using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. The Akt antibody confirms silencing of protein expression while the p42 MAP Kinase (Erk2) antibody was used to control for loading and specificity of Akt siRNA (A). Phospho-GSK-3β (Ser9) Antibody #9336 was used to confirm downstream pathway inhibition (B).
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. Akt antibody confirms silencing of Akt expression, while the p42 MAP kinase (Erk2) antibody is used to control for loading and specificity of Akt siRNA.
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (50 ng/ml) for the indicated times, using Phospho-Akt (Ser473) Antibody #9271 (upper) or Akt Antibody (lower).
Confocal immunofluorescent images of C2C12 cells showing nuclear and cytoplasmic localization with Akt Antibody (left, red) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin.
Flow cytometric analysis of Jurkat cells, untreated (blue) or LY294002-treated (green), using Akt Antibody compared to a nonspecific negative control antibody (red).