Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181851 at 1/60 dilution (lane 1). Negative control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes : Anti-BarX1 antibody [EPR14120] (ab181851) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution