ab112521 staining BCA1 in Rat brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal(1/1000) was used as the secondary antibody.See Abreview
All lanes : Anti-BCA1 antibody (ab112521)Lane 1 : Recombinant human BCA1 protein at 0.01 µgLane 2 : Recombinant human BCA1 protein at 0.005 µgLane 3 : Recombinant human BCA1 protein at 0.0025 µg
ab112521 staining BCA1 in human lung cancer tissue.