ICC/IF image of ab101704 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101704 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
IHC ( paraffin section) on rat spinal cord using ab101704 at 1 : 200 dilution incubated overnight at room temperature, developed with DAB/Ni and counterstained with hematoxylin.
ab101704 staining BDKRB2 in human tendon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with peroxidase blocking buffer for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000) for 1 hour.See Abreview