Anti-BDNF antibody
| Name | Anti-BDNF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6201 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | DB ICC/IF ICC/IF ELISA B/N WB IHC-F |
| Species Reactivities | Mouse, Rat, Horse, Human, Pig |
| Antigen | Recombinant full length protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | BDNF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-BDNF antibody (ab6201)Lane 1 : Purified rHBNDF at 0.05 µgLane 2 : Mouse brian lysate at 15 µgObserved band size : 14,28 kDa (why is the actual band size different from the predicted?)
ab6201 BDNF antibody immunostaining in the ventral horn of the spinal cord. Protocol: free floating, PFA/picric acid perfusion fixed rat coronal sections (30 microns) were incubated overnight in ab6201 (1/500) followed by 1 night of postfixation). Immunofluorescence was visualised with TSA amplification.
ab6201 BDNF antibody immunostaining BDNF-containing hippocampal neurons. Protocol: PFA(only)-fixed free floating coronal rat brain sections (30 microns) were incubated for 3 days with ab6201 at 1/100 followed by direct immunofluorescence detection (alexa488; 1/1000). We recommend using an amplification IHC protocol following PFA/picric acid fixation of brain tissue for optimising BDNF detection using ab6201
ICC/IF image of ab6201 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6201, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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