Anti-BDNF antibody
| Name | Anti-BDNF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab46176 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A peptide from the N-terminal region of mature mouse BDNF conjugated to a carrier protein to enhance immunological response |
| Description | Rabbit Polyclonal |
| Gene | BDNF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab46176 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46176 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-BDNF antibody (ab46176) at 1/1000 dilutionLane 1 : Rat Brain at 10 µlLane 2 : negative control
Product References
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