All lanes : Anti-BDNF antibody [EP1293] (ab108383) at 1/1000 dilutionLane 1 : C6 cell lysate at 10 µgLane 2 : Fetal brain lysate at 10 µgLane 3 : Human hippocampus lysate at 10 µgLane 4 : Recombinant BDNF protein at 10 ng
Immunofluorescent staining of PC12 cells using ab108383 at a dilution of 1/100.
ab108383 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab108383 at a 1/100 dilution for 14 hours at 4°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.See Abreview