Anti-beta Actin antibody
| Name | Anti-beta Actin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16039 |
| Prices | $390.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IP |
| Species Reactivities | Mouse, Human, Rat, Sheep, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Pig, Xenopus, Chimpanzee, Drosophila, Zebrafish, Xenopus |
| Antigen | Synthetic peptide: APRAVFPS , corresponding to amino acids 26-33 of beta Actin |
| Description | Rabbit Polyclonal |
| Gene | ACTB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-beta Actin antibody (ab16039) at 1 µg/mlLane 1 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab16039 at 1/100 dilution staining Human beta Actin in Leiomyoma by Immunohistochemistry (FFPE-sections)
![beta Actin was immunoprecipitated using 0.5mg SK-N-SH whole cell extract, 5µg of Rabbit polyclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 42kDa; beta Actin](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13591_ab16039-195130-IPV021ab1603920min.jpg)
beta Actin was immunoprecipitated using 0.5mg SK-N-SH whole cell extract, 5µg of Rabbit polyclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 42kDa; beta Actin
Product References
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