Anti-beta Actin antibody [AC-15]
| Name | Anti-beta Actin antibody [AC-15] |
|---|---|
| Supplier | Abcam |
| Catalog | ab82618 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AC-15 |
| Applications | FC ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Rat, Human, Sheep, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Pig, Hamster |
| Antigen | Synthetic peptide corresponding to beta Actin aa 2-15 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Mouse Monoclonal |
| Gene | ACTB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab82618 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82618, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13643_ab82618-2-ab82618FC.jpg)
Overlay histogram showing HeLa cells stained with ab82618 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82618, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
ICC/IF image of ab82618 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82618, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
In vivo and in vitro analysis of age-associated changes and somatic cellular - In vivo and in vitro analysis of age-associated changes and somatic cellular
Berkenkamp B, Susnik N, Baisantry A, Kuznetsova I, Jacobi C, Sorensen-Zender I, Broecker V, Haller H, Melk A, Schmitt R. PLoS One. 2014 Feb 4;9(2):e88071.
Variable expressivity of ciliopathy neurological phenotypes that encompass - Variable expressivity of ciliopathy neurological phenotypes that encompass
Abdelhamed ZA, Wheway G, Szymanska K, Natarajan S, Toomes C, Inglehearn C, Johnson CA. Hum Mol Genet. 2013 Apr 1;22(7):1358-72.