Anti-beta Actin antibody [GT5512]

• Supplier: Abcam
• Catalog: ab184220
• Prices: $387.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: GT5512
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Rabbit, Goat, Human, Yeast, Drosophila, Zebrafish, Sheep, Chicken, Guinea Pig, Bovine, Chimpanzee, Monkey
• Antigen: Synthetic peptide within Human beta Actin aa 315-374 (C terminal)
• Description: Mouse Monoclonal
• Gene: ACTB
• Conjugate: Unconjugated

Product images

Anti-beta Actin antibody [GT5512] (ab184220) at 1/10000 dilution + Rabbit blood lysate/extract at 30 µg

Anti-beta Actin antibody [GT5512] (ab184220) at 1/10000 dilution + goat blood lysate/extract at 30 µg

All lanes : Anti-beta Actin antibody [GT5512] (ab184220) at 1/10000 dilutionLane 1 : 293T whole cell lysate/extract at 20 µgLane 2 : 293T whole cell lysate/extract at 10 µgLane 3 : 293T whole cell lysate/extract at 5 µgLane 4 : 293T whole cell lysate/extract at 1 µg

Anti-beta Actin antibody [GT5512] (ab184220) at 1/1000 dilution + Zebrafish lysate/extract at 30 µg

All lanes : Anti-beta Actin antibody [GT5512] (ab184220) at 1/20000 dilutionLane 1 : Jurkat whole cell lysate/extractLane 2 : Raji whole cell lysate/extractLane 3 : 293T whole cell lysate/extractLane 4 : A431 whole cell lysate/extractLane 5 : HeLa whole cell lysate/extractLane 6 : HepG2 whole cell lysate/extractLane 7 : H1299 whole cell lysate/extractLane 8 : HCT116 whole cell lysate/extractLane 9 : MCF7 whole cell lysate/extractLane 10 : NT2D1 whole cell lysate/extractLane 11 : PC3 whole cell lysate/extractLane 12 : U87-MG whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-beta Actin antibody [GT5512] (ab184220) at 1/10000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : NIH3T3 whole cell lysate/extractLane 3 : Mouse brain whole cell lysate/extractLane 4 : PC12 whole cell lysate/extractLane 5 : Rat brain whole cell lysate/extractLysates/proteins at 30 µg per lane.

Anti-beta Actin antibody [GT5512] (ab184220) at 1/1000 dilution + yeast lysate/extract at 10 µg

Anti-beta Actin antibody [GT5512] (ab184220) at 1/1000 dilution + drosophila lysate/extract at 30 µg

Immunofluorescent analysis of HeLa cells (fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min) labeling beta Actin protein with ab184220 diluted at 1:500 (Green). Counterstained with Hoechst 33342 (Blue).